Isoflurane Exposure Influence on Sleep Architecture in a Rat Model of Alzheimer’s Disease

Author: Kreuzer M, et al.

Anesthesia & Analgesia, August 29, 2025. doi:10.1213/ANE.0000000000007717

This experimental study investigated the effects of isoflurane anesthesia on sleep and recovery patterns in a rat model of Alzheimer’s disease (TgF344-AD). The researchers used EEG and EMG recordings in 8 transgenic rats and 7 age-matched controls to monitor WAKE, NREM, and REM states before and after a one-hour isoflurane exposure.

Alzheimer-model rats took significantly longer to ambulate after anesthesia compared with controls (1256 seconds vs. 799 seconds, P = .038). During the 5-hour recovery window, no major differences were noted in vigilance states. However, in the subsequent active phase, control rats displayed increased sleep, while Alzheimer-model rats showed heightened wakefulness, fragmented sleep patterns, and relative hyperactivity.

Key Takeaways

  • Isoflurane delayed ambulation in Alzheimer-model rats compared with controls.

  • No immediate differences in WAKE, NREM, or REM proportions were observed.

  • Alzheimer-model rats experienced fragmented sleep and relative hyperactivity post-anesthesia.

  • Findings suggest Alzheimer pathology reduces resilience to anesthetic-induced brain state changes.

Thank you to Anesthesia & Analgesia for publishing this important research on anesthesia, sleep, and Alzheimer’s disease.

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